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Effect of budesonide on MDR1 mRNA stability in Caco-2 cells. Cells were treated with 25 μM budesonide or vehicle, whereas transcription was inhibited with actinomycin D (5 μg ml−1). mRNA expression was determined by quantitative real-time PCR after 0, 4, 8, 24 and 48 h incubation. Each data value is expressed relative to the expression at 0 h. Data represent mean±s.e.m. (n=3).
