Figure 6.

H₂O₂ reduces steady-state α1 mRNA levels. RASM were incubated for 12 and 24 h with 500 μM of H₂O₂ (12 and 24) or the corresponding vehicle (con). Total RNA was isolated, and cDNA was prepared as described in the Methods. α1 mRNA levels determined by semiquantitative RT-PCR (a) and quantitative real-time PCR (b). (a) Photograph depicts an ethidium bromide-stained agarose gel with the RT-PCR products for sGC α1 and GADPH. W=water, used as control in the PCR. (b) The bar graph shows the relative decrease of α1 mRNA levels in cells treated with H₂O₂ for 12 h compared to control in quantitative real-time RT-PCR.