Table 3.

Inhibition of [³⁵S]TBPS binding by TBPS, picrotoxin, GABA, loreclezole and pentobarbital in postmortem human and rat cortex and cerebellum membranes

	Human brain				Rat brain
	Cortex		Cerebellum		Cortex		Cerebellum
	IC50 (μM)	Hill slope	IC50 (μM)	Hill slope	IC50 (μM)	Hill slope	IC50 (μM)	Hill slope
TBPS	0.054±0.009*	0.86±0.15	0.046±0.012*	1.18±0.23	0.021±0.005	0.91±0.07	0.022±0.005	0.95±0.02
Picrotoxin	0.31±0.03*	0.84±0.02	0.43±0.06*	0.87±0.11	0.18±0.04	0.85±0.03	0.18±0.03	0.87±0.03
GABA	0.90±0.07	1.35±0.05	0.65±0.11	1.24±0.17	1.13±0.15	1.56±0.14	0.90±0.08	1.21±0.08
Loreclezole	21±7*	0.88±0.09	12±4*	0.90±0.11	8.4±1.8	0.83±0.06	5.0±0.9	0.99±0.08
Pentobarbital	177±42*	1.11±0.13	110±25*	1.08±0.05	64±9	1.14±0.05	58±10	1.11±0.06

Abbreviations: GABA, γ-aminobutyric acid; [³⁵S]TBPS, [³⁵S]t-butylbicyclophosphorothionate; TBPS, t-butylbicyclophosphorothionate.

Data shown are mean±s.e.m. (n=3–4).

^*P<0.05; comparison with corresponding rat tissue using an unpaired t-tests.