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Mechanisms of impaired NO production in TA: Western immunoblotting showing the protein expression of eNOS, phospho-eNOSSer1177, Akt, phospho-AktThr308 and β-actin in the (a) TA and (b) AA from control and Marfan mice at different ages. Expression of β-actin served as loading control. Because of the limited aortic samples from each mouse, TA and AA were pooled from each group of animals at different ages.
