Figure 3.

Activation of ERK, p38 MAP kinase and IκBα degradation by TNFα, IL-1β and trypsin in HUVECs. Cells were stimulated with TNFα (10 ng ml⁻¹), IL-1β (10 ng ml⁻¹) and trypsin (30 nM) for the times and at the concentrations outlined. Cell lysates were assessed by Western blotting for p38 MAP kinase (a) and ERK activation (b) using specific anti-phospho antibodies, IκBα levels using an anti-IκBα antibody (c), or total ERK and p38 MAP kinase (d) as outlined in the Materials and methods. Blots were semi-quantified by densitometry. Each blot is representative of at least three others. Statistical analysis was performed by Dunnett's test for multiple comparisons. ^**P<0.01 vs control; ^*P<0.05 vs control.