Table 1. Origin, properties of Ralstonia solanacearum (GM1000) genes used in this study as plasmid or genomic donor DNA; plasmid transformation frequencies and number of Chi-like sequences detected within the 2 kb long DNA positions.
| Accession number | Gene location | Gene Acronym | Gene function | Putative Origin | Plasmid transformation frequencies | Number of Chi like 5′ cGCCGAAc 3′ within 2 Kb DNA fragment | Acronym for the targeted genomic positionsa (TCPb versus TMP) |
| RSc3437 | 3710105 | vsr | Avirulence | Caulobacter crescentus (α-proteobacteria) | 1.29+/−0.20×10−6 | 1 | TCP15 |
| RSc3252 | 3506740 | - | - | Chlorobium .tepidum (green sulfurous bacteria) | 2.40+/−0.15×10−6 | 3 | TCP14 |
| RSc1815 | 1981107 | - | - | Xanthomonas campestris (γ-proteobacteria) | 2.60+/−0.09×10−6 | 3 | TCP8 |
| RSc0558 | 602739 | pilA | Virulence | Pseudomonas aeruginosa (γ-proteobacteria) | 1.14+/−0.26×10−6 | 1 | TCP4 |
| RSp0313 | 411136 | mexC | - | Acinetobacter baumannii (γ-proteobacteria) | 1.38+/−0.44×10−7 | 0 | TMP1 |
| RSc0828 | 869969 | tIS14b | IS | Element of external origin | 6.08+/−1.66×10−6 | 3 | TCP5 |
| RSc1921 | 2100591 | - | Phage | Element of external origin | 3.59+/−0.34×10−6 | 1 | TCP9 |
| RSc2585 | 2791556 | tn | Transposon | Element of external origin | 8.27+/−1.03×10−6 | 1 | TCP12 |
| RSc0458 | 489163 | ubiE | House keeping | R. solanacearum (β-proteobacteria) | 4.99+/−2.47×10−8 | 0 | TCP2 |
| RSc2191 | 2373410 | purD | House keeping | R. solanacearum (β-proteobacteria) | 4.12+/−1.43×10−6 | 1 | TCP10 |
| RSc2341 | 2538801 | ftsK | House keeping | R. solanacearum (β-proteobacteria) | 5.27+/−0.14×10−7 | 5 | TCP11 |
| RSc3023 | 3244061 | rpsG | House keeping | R. solanacearum (β-proteobacteria) | 1.40+/−0.29×10−7 | 3 | TCP13 |
| RSc0551 | 596177 | recA | House keeping | R. solanacearum (β-proteobacteria) | 1.66+/−0.26×10−5 | 6 | TCP3 |
| RSc1120 | 1176593 | comA | House keeping | R. solanacearum (β-proteobacteria) | 6.27+/−0.91×10−7 | 4 | TCP6 |
| RSc1151 | 1207216 | mutS | House keeping | R. solanacearum (β-proteobacteria) | 1.00+/−0.25×10−5 | 3 | TCP7 |
| RSc0171 | 192394 | - | Putative gene | Putative gene | 1.45+/−0.28×10−6 | 1 | TCP1 |
| RSp1328 | 1678048 | - | Putative gene | Duplication | 2.22+/−0.66×10−6 | 3 | TMP2 |
| RSp0877 | popA | Virulence | R. solanacearum (β-proteobacteria) | nd | nd | TMP3 |
a
TOPO recombinant plasmids with R. solanacearum inserts labelled by an aacC3-IV gene-cassette conferring resistance to gentamycin (GmR)
b
Targeted Chromosomal Position (versus Megaplasmid)
nd, not determined