Table 1.

Oligonucleotides of primers for PCR

Gene product	Sense	Antisense	Predicted size (bp)
nNOS	5′-GCGGA GCAGA GCGGC CTTAT-3′	5′-TTTGGT GGGAG GACCG AGGG-3′	240
eNOS	5′-CCGCA CTTCT GTGCC TTTGC TC-3′	5′-GCTCG GGTGG ATTTGC TGCTCT-3′	360
M2 mAChR	5′-GATGA AAACAC AGTT TCCAC TTC-3′	5′-ATGAT GAAAGC CAACA GGATA GCC-3′	280
G3PDH	5′-ACCAC AGTCCA TGCCAT CAC-3′	5′-TCCAC CACCC TGTTG CTGTA-3′	452

Abbreviations: eNOS, endothelial nitric oxide synthase; G₃PDH, glyceraldehyde-3-phosphate dehydrogenase; M₂ mAChR, M₂ muscarinic acetylcholine receptor; nNOS, neuronal nitric oxide synthase; PCR, polymerase chain reaction.

Neuronal (nNOS) and endothelial (eNOS) nitric oxide synthase (Qi et al., 2001); M₂ mAChR (Steel and Buckley, 1993); G₃PDH (Bishop-Bailey et al., 1997).