Figure 3.
F15063 efficaciously activates cloned human and native rat 5-HT1A receptors. (a) Stimulation by F15063 of G-protein activation determined using [35S]GTPγS binding at recombinant human (HeLa-h5-HT1A) and (b) native rat hippocampal 5-HT1A receptors. (c) Inhibition of cAMP accumulation in HeLa-h5-HT1A cells. (d) stimulation of ERK1/2 phosphorylation at h5-HT1A receptors expressed in CHO cells. Data are expressed as percentage of the effect induced by a saturating concentration of 5-HT (10 μM). Values are mean±s.e.m. from three experiments performed in triplicate or in duplicate. For comparison, the dotted line represents results obtained under the same conditions for (±)8-OH-DPAT (Newman-Tancredi et al., 2005; Bruins Slot et al., 2006). Data from these experiments are shown in Table 6.