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. 2007 Apr 2;151(4):518–529. doi: 10.1038/sj.bjp.0707237

Figure 4.

Figure 4

BEO reduces cell death induced by NMDA in SH-SY5Y cultures. (a) BEO (final concentrations in medium: 0.0005, 0.005 and 0.01%) was added to SH-SY5Y cultures 60 min before application of 1 mM NMDA and cell death was assessed by trypan blue exclusion assay 24 h later; no protection against NMDA-induced cell death was produced by BEO at the lowest concentration (0.0005%). (b) Ethanol (ET, 0.04 and 0.08%), the vehicle used to dissolve BEO, does not affect 1.0 mM NMDA-induced cell death; these concentrations of ethanol correspond to those found in 0.005 and 0.01% BEO. Each value is the mean±s.e.m. of 4–12 experiments. *P<0.05 and ***P<0.001 vs control, respectively; ###P<0.001 vs NMDA given alone (ANOVA followed by Tukey–Kramer multiple comparisons test).