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. 2007 Apr 2;151(4):518–529. doi: 10.1038/sj.bjp.0707237

Figure 7.

Figure 7

LY294002 concentration dependently reversed the neuroprotection afforded by BEO against NMDA-induced cell death. SH-SY5Y cells were treated with LY294002 (0.002–20 μM), a specific inhibitor of PI3K, for 30 min before the addition of BEO (0.01%, added 1 h before NMDA) and of 1 mM NMDA for 24 h and, then, cell death assessed by means of trypan blue staining. SH-SY5Y cells were treated with LY294002 (20 μM) for 30 min before exposure to 1 mM NMDA for 24 h. LY294002, given alone, did not affect NMDA-induced cell death. Each value is the mean±s.e.m. of 4–6 experiments. #P<0.05, ##P<0.01 and ###P<0.001 vs NMDA, respectively; $P<0.05, $$P<0.01 and $$$P<0.001 vs BEO+NMDA, respectively (ANOVA followed by Tukey–Kramer multiple comparisons test).