Figure 5.

The role of Rap1 in 2-DG uptake in VSMC. (a) Forty-eight hours after transfection with the siRNA against Rap1, cell lysates were prepared and subjected to SDS-PAGE and immunoblotting with and anti-Rap1 antibody, anti-Rac antibody or anti-α-smooth muscle (SM) actin antibody. (b) Rap1-silenced VSMC were serum-starved for 4 h and then stimulated with adrenaline (Adr, 10 μM) for 1 h. Then uptake of 2-DG by the VSMC was measured. Each value represents the mean±s.d. of three independent experiments in triplicate. ^*P<0.05.