Figure 5.

Histograms showing the EDHF-mediated hyperpolarization (left panels) and relaxation (right panels) in the presence of the NOS inhibitor L-NAME (100 μM) and either: (a) the thromboxane synthase inhibitor, furegrelate (10 μM), or (b) the cyclo-oxygenase inhibitor indomethacin (10 μM). Also shown are the effects of the IK_Ca inhibitor, TRAM-34 (1 μM), the SK_Ca inhibitor apamin (50 nM) and the BK_Ca inhibitor iberiotoxin (IbTx; 100 nM). Only combined application of TRAM-34, apamin and iberiotoxin fully blocked the EDHF response in the presence of either furegrelate or indomethacin. ^*P<0.05, ^**P<0.01 and ^***P<0.001 indicate a statistically significant difference from control.