Table 1.
Effect of temperature and assay buffer on the estimated pKL, nH and Bmax of [3H]clobenpropit in guinea-pig cerebral cortex membranes
| Temperature (°C) |
Buffer A |
Buffer ACa |
||||
|---|---|---|---|---|---|---|
| pKL | Bmax | nH | pKL | Bmax | nH | |
| 30 | 10.15±0.04 | 4.00±1.17 | 0.93±0.02 | ND | ND | ND |
| 21 | 10.40±0.08 | 3.80±0.97 | 1.08±0.09 | 9.77±0.20 | 3.28±0.65 | 1.20±0.05 |
| 12 | 10.38±0.07 | 4.06±0.55 | 0.90±0.03 | 9.69±0.18 | 4.02±0.83 | 1.05±0.09 |
| 4 | 10.57±0.03 | 4.00±0.48 | 1.11±0.06 | 9.66±0.27 | 4.04±0.68 | 1.13±0.12 |
Abbreviations: ND, not determined.
pKL, Bmax (fmol mg−1) and nH values were obtained by fitting saturation data to the Hill equation.
Data are the mean±s.e.m. of three experiments.