Table 2.
Effect of CA feeding with or without co-treatment with PCN on hepatic nuclear receptor expression
|
Relative levels of PXR, FXR and CAR mRNA |
||||||
|---|---|---|---|---|---|---|
|
PXR+/+ |
PXR−/− |
|||||
| Control | CA | CA+PCN | Control | CA | CA+PCN | |
| PXR | 100±26 | 346±42a | 300±21a | — | — | — |
| FXR | 100±7 | 359±42a | 158±19 | 156±39 | 423±98c | 279±69 |
| CAR | 100±27 | 58±27 | 77±25 | 156±56 | 335±53b,c | 223±57 |
Abbreviations: CA, cholic acid; CAR, constitutive androstane receptor; FXR, farnesoid X receptor; PCN, 5-pregnen-3β-ol-20-one-16α-carbonitrile; PXR, pregnane X receptor.
PXR+/+ and PXR−/− mice (n=4–6) were fed a 1% CA-supplemented diet and treated with the PXR activator PCN or corn oil control. Levels of hepatic nuclear receptor mRNA were measured by RT-PCR. Values are presented as a percentage of the mRNA levels in PXR+/+ control mice (±s.e.m.).
a
P<0.05 vs PXR+/+ control.
b
P<0.05 vs PXR+/+ fed CA.
c
P<0.05 vs PXR−/− control.