Figure 3.
Typical traces showing the effect of MβCD on IKCa- and SKCa-dependent vasorelaxation in rat mesenteric arteries. Arteries were mounted on a pressure myograph and pressurized to 70 mm Hg. Arteries were pre-incubated for 45 min with 5 mM MβCD and either (a) 100 nM apamin or (b) 10 μM TRAM-34. Arteries were constricted with 5–30 nM phenylephrine (PE) and 0.3–3 μM ACh applied. Full relaxation was induced with 100 μM papaverine. Mean data showing relaxations to ACh in the presence of (c) apamin and (d) TRAM-34 before and after incubation with MβCD (n=4).