Table 2.

The effect of different amounts of WT or P246A receptor DNA on function (pEC₅₀ and E_max)

	WT			P246A
	pEC50	Emax	n	pEC50	Emax	n
250 ng	9.96±0.10	363.7±91.0	3	10.03±0.42	485.0±59.2	3
125 ng	9.64±0.21	314.0±64.0	3	9.80±0.12	391.4±44.2	3
62.5 ng	9.61±0.04	271.2±71.6	3	9.60±0.18	347.1±22.8*	3
31.3 ng	9.62±0.24	205.4±39.9**	3	9.39±0.26	273.2±36.4**	3
15.6 ng	9.58±0.32	168.8±28.2**	3	9.28±0.59	214.6±15.5**	3

Abbreviations: WT, wild type.

^*P<0.05,

^**P<0.01

^*P<0.05, ^**P<0.01 vs WT 250 ng or P246A 250 ng as appropriate by one-way ANOVA followed by Dunnett's multiple comparison test.

E_max values are the maximum asymptote of concentration–effect curves and are concentrations (nM) of cAMP.