Table 1.
Primers
| Genea | GenBank accession number | Primer |
|---|---|---|
| Mat1a | NM_133653 | F: 5′-CTG AGG CGC TCT GGT GTC-3′ |
| R: 5′-TCC TGC ATG TAC TGA ACT GTT ACC-3′ | ||
| Mat2a | NM_145569 | F: 5′-CCG AGT CTG TAG GGG AAG GT-3′ |
| R: 5′-GGT CTT GTT GAA GGT GTG CAT-3′ | ||
| Gnmt | NM_010321 | F: 5′-GCT GGA CGT AGC CTG TGG-3′ |
| R: 5′-CAC GCT CAT CAC GCT GAA-3′ | ||
| Ahcy | NM_016661 | F: 5′-CTG TTG GGG TTC ACT TCC TG-3′ |
| R: 5′-ACA TTC AGC TTG CCC AGG T-3′ | ||
| Bhmt | NM_016668 | F: 5′-ACG TGG ACT TCC TCA TTG CAG AGT-3′ |
| R: 5′-TGC TAC GGG CTT ACC AGA TGC TTT-3′ | ||
| Mtr | XM_138431 | F: 5′-GCA GAT GTG GCC AGA AAA G-3′ |
| R: 5′-GCC ACA AAC CTC TTG ACT CC-3′ | ||
| Mthfr | NM_010840 | F: 5′-AGCTTGAAGCCACCTGGACTGTAT-3′ |
| R: 5′-AGACTAGCGTTGCTGGGTTTCAGA-3′ | ||
| Cth | NM_145953 | F: 5′-GAG TCT GGC TGA GCT TCC A-3′ |
| R: 5′-CGA GGG TAG CTC TGT CCT TC-3′ | ||
| Cbs | NM_144855 | F: 5′-CGC ACA GGA AGG ACT GCT A-3′ |
| R: 5′-AGC CTT CAC AGC CAC AGC-3′ | ||
| Rn18s | X00686 | F: 5′-AAA TCA GTT ATG GTT CCT TTG GTC-3′ |
| R: 5′-GCT CTA GAA TTA CCA CAG TTA TCC AA-3′ |
a
LocusLink official symbol followed by official gene name. Mat1a: methionine adenosyltransferase I, alpha; Mat2a: methionine adenosyltransferase II, alpha; Gnmt: glycine N-methyltransferase; Ahcy: S-adenosylhomocysteine hydrolase; Bhmt: betaine-homocysteine methyltransferase; Mtr: 5-methyltetrahydrofolate-homocysteine methyltransferase; Mthfr: 5,10-methylenetetrahydrofolate reductase; Cth: cystathionase (cystathionine γ-lyase); Cbs: cystathionine -βsynthase; Rn18s: 18S RNA.