Figure 4.

(A) Northern blot analysis of syt X expression. Poly(A)⁺ RNA from parietal cortex, hippocampus, and piriform cortex at 0, 3, 6, 12, and 24 h after kainic acid-induced seizures was probed with syt X. The probe identifies a single message that is 8.0 kb in length. The blot was also probed with a β-actin oligonucleotide for the purpose of quantitation. (B) In situ hybridization of rat brain sections using an oligonucleotide probe specific for the syt X gene. Coronal section control and 6-h kainic acid-treated rat brain. Examination of control and kainic acid-treated animals revealed that syt X is expressed in the dentate granule cells of the hippocampus and layer II of the piriform cortex.