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Influence of the Gs-selective Bpa(7)-Ucn I at fixed concentrations on the stimulation by sauvagine of the [35S]-GTPγS binding in HEK-rCRF1 cell membranes expressing selectively Gi activity. Data points represent the mean±s.d. of triplicates and were fitted by nonlinear regression. From the EC50 values obtained, a Schild plot for competitive antagonism was drawn (inset). HEK-rCRF1, HEK293 cells stably transfected with rCRF1.
