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. 2007 May 21;151(6):749–757. doi: 10.1038/sj.bjp.0707254

Figure 2.

Figure 2

Effect of EtOH on NMDA-activated current in cells expressing NR2A(F637) mutant subunits. (a) Records are currents activated by 10 μM glutamate (Glu) and 50 μM glycine in the absence and presence of 100 mM EtOH in human embryonic kidney (HEK) 293 cells coexpressing NR1 and wild-type (WT) NR2A or various NR2A(F637) substitution mutant subunits. (b) Concentration–response curves for EtOH inhibition of current activated by 10 μM glutamate (Glu) and 50 μM glycine in HEK 293 cells coexpressing NR1 and wild-type NR2A (WT) or various NR2A(F637) substitution mutant subunits. Data points are means of five to 12 cells and curves shown are the least-squares fits to the equation given in Materials and methods. (c) Bar graph shows IC50 values for EtOH in cells expressing the various NR2A(F637) mutant subunits. Asterisks indicate IC50 values that differed significantly (ANOVA and Dunnett's test; *P<0.05, **P<0.01) from the IC50 for WT NR1/NR2A subunits.