Figure 5.

Relation between NMDA-receptor agonist potency and desensitization in NR2A(F637) mutant subunits. (a) Graph plots steady-state vs peak EC₅₀ values for glutamate in the various NR2A(F637) mutant subunits. Data points are labeled with the substituted amino acid for the various mutants at NR2A(F637). The line shown is the least squares fit to the data; glutamate peak and steady-state EC₅₀ were significantly correlated (R²=0.900, P<0.0001). (b) Graph plots steady-state and peak log EC₅₀ values for glutamate vs the I_ss:I_p of glutamate-activated current in the various NR2A(F637) mutant subunits. Maximal I_ss:I_p for glutamate was not correlated with either peak glutamate log EC₅₀ (R²=0.340, P>0.05) or steady-state glutamate log EC₅₀ (R²=0.075, P>0.05). The lines shown are the least squares fits to the data.