Skip to main content
. 2007 May 29;151(6):816–827. doi: 10.1038/sj.bjp.0707269

Figure 2.

Figure 2

Matrigel- and non-coated plastic-cultured cells responded differently to endothelin-1 (ET-1) and platelet-derived growth factor (PDGF). Myofibroblasts were cultured on either Matrigel or non-coated plastic dishes and starved overnight. Changes in intracellular Ca2+ concentration ([Ca2+]I) were measured using Ca2+ fluorescent dye fura-2. Results are shown as the area under the [Ca2+]i-time curve (AUC: 0–2 min) for 100 μM adenosine 5′-triphosphate (ATP) (a), 100 nM ET-1 (b) and 10 ng ml−1 PDGF (c). Cells were pretreated with 3 μM BQ-123 for 3 min before stimulation with ET-1 in (d). Data are presented as mean±s.e.m. of 17–43 cells from 4–5 separate experiments. **P<0.01.