Figure 4.

Effect of nicardipine on the increase in intracellular Ca²⁺ concentration ([Ca²⁺]_i) due to 10 μM adenosine 5′-triphosphate (ATP), 100 nM endothelin-1 (ET-1) and 10 ng ml⁻¹ platelet-derived growth factor-BB (PDGF-BB) in myofibroblasts cultured on either Matrigel (a and b) or non-coated plastic dish (c and d). Results are shown as the area under the [Ca²⁺]_i-time curve (AUC: 3–4 min). Myofibroblasts were pretreated with 100 nM nicardipine for 3 min before stimulation with these agonists. Data are presented as the mean±s.e.m. of 13–40 cells from 4 separate experiments. ^**P<0.01.