Figure 3.

The effect of the antioxidant PDTC and catalase on Aβ-induced NF-κB activation. Granule cells were incubated for 45 min with 100 nM Aβ alone [a and b, Aβ-(1–40); c and d, Aβ-(25–35)] or with Aβ and 100 μM PDTC, which was added to cell cultures 10 min before Aβ [e and f, Aβ-(1–40); g and h, Aβ-(25–35)], or with 100 nM Aβ plus 400 μg/ml catalase, which was added to cells 1 h before the Aβ peptide [i and j, Aβ-(1–40); k and l, Aβ-(25–35)]. Cell cultures were analyzed by indirect immunofluorescence for an increase of α-p65 mAb immunoreactivity (a, e, i, c, g, k) and by nuclear DAPI-staining (b, f, j, d, h, l).