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Northern blot analysis of RNA in 3T3-L1 cells. Total RNA was isolated from the 3T3-L1 cells cultured in the presence (left two lanes) or absence of (right two lanes) 10 μm troglitazone, and 20 μg of RNA samples were examined by Northern blot analysis. Hybridization was carried out over night in the presence of 32P-labelled carboxyl terminal of mouse LPL (405 bp) cDNA.
