Figure 4.

Effect of RWPs and several authentic RWPs on the PDGF_AB-induced formation of ROS in VSMCs. VSMCs were exposed to either solvent (0.1% DMSO), RWPs (30 μg ml⁻¹) or an authentic RWP (30 μM) for 30 min before the addition of PDGF_AB. After a 1-h incubation period, the generation of intracellular ROS was assessed using DCF and expressed in arbitrary units as a fold increase of the signal obtained with control cells. Results are shown as mean±s.e.m. of three different experiments performed in triplicate. ^*versus control, ^#versus PDGF_AB treatment alone. SOD: superoxide dismutase.