Figure 2.
Increase in open probability (Po) of the single BKCa channels by intracellular application of sulphatide at inside-out patches. (a) Current traces before (control) and after successive applications of sulphatide (1.25–10 μM) to the intracellular face of channels on the same patch. (b) Dose–response curve for the effect of sulphatide on the Po of the BKCa channels. Solid line is the best fit to the Hill equation (see text). (c) Current traces before, during application and washout of 10 μM sulphatide (representative for six cells). Channel openings are shown as an upward deflection. The arrows indicate the level at which all the channels are at their closed state.