Table 1.

Neutrophil – macrophage interactions

Brain region	% of total PMN IR cells colocalized with ED1		% of total PMN IR cells not colocalized with ED1
	ET-1+water	ET-1+AM-36	ET-1+water	ET-1+AM-36
Hindlimb cortex	24±1.7	3±2.5***	76±1.7	97±2.5***
Forelimb cortex	27±0.8	16±0.6***	73±0.8	85±0.9**
Striatum	25±0.7	11±1.2***	75±3.2	89±1.3*
Motor cortex	31±2.0	19±2.2***	69±2.0	75±4.3
Parietal cortex	29±1.9	16±3.3**	71±2.0	84±3.3**
Barrel cortex	32±2.0	25±2.8*	68±2.0	75±2.8

Changes in percentage following AM-36 administration in the number of PMN IR cells colocalized or not colocalized with ED1 in different cerebral regions. AM-36 treatment significantly reduced the total number PMN IR cells that were colocalized with ED1, by approximately 10–15% when compared with vehicle-treated rats. Conversely, the percentage of the total number of PMN IR cells not colocalized with ED1 was increased by approximately 10–15% following AM-36 treatment in all cerebral regions except the motor cortex, where no significant difference existed. Data is presented as mean±s.e.m.

^*P<0.05

^**P<0.001

^***P<0.0001 AM-36 compared with vehicle-treated rats (Kruskal–Wallis ANOVA with Dunn's post-test). n=5–6 in each group.