Figure 4.
Effects of isoflurane on recombinant KATP channels expressed in HEK293 cells. Representative changes of Kir6.1/SUR2B, Kir6.2/SUR2A and Kir6.2/SUR2B channel currents after isoflurane (Isof), pinacidil (Pina) and glibenclamide (Glib) and the summarized data are shown in (a–c), respectively. The membrane potential was held at −40 mV and the same ramp-pulse protocol as that in Figure 3 was used to record the quasi-steady-state membrane current. The current level at 0 mV was measured and then the current density was calculated. Values are expressed as mean±s.e.m. of nine cells. Statistical analyses of the data were conducted using two-way ANOVA combined with Fisher's post hoc test.