Figure 5.

(a) Effects of AM10 on single KATP channel activity. The transmembrane potential was clamped at −40 mV (inside negative). Each trace shows 30 s of recording before (upper trace), during (middle trace) and after the washout (lower trace) of AM10 (100 nM) in the presence of trypsin (100 μg ml⁻¹) in the bath solution. (b) Effects of AM10 on the K⁺ current resulting from the expression of Kir6.2ΔC36 in tsA201 cells. Representative traces of the current elicited by a 200 ms hyperpolarization of 100 mV from the holding potential of 0 mV in control condition (K⁺ equimolar; lower trace), in the presence of Ba²⁺ (2 mM; upper trace) or in the presence of AM10 (100 nM; middle trace). (c) Effects of AM10 on single Kir6.2ΔC36 channel activity. The transmembrane potential was clamped at −40 mV (inside negative). Each trace shows 30 s of recording in control condition (upper trace) and after application of AM10 (100 μM) (middle trace) and ATP (10 mM) (lower trace) in the bath solution. (a, b and c) Traces representative of six, 10 and three experiments, respectively.