Skip to main content
. 2006 Oct 16;149(7):888–897. doi: 10.1038/sj.bjp.0706899

Figure 4.

Figure 4

DMAsIIIG is an unstable chemical; DMAsIIIG-induced cytotoxicity was reduced by preincubation. (a) DMAsIIIG alone was preincubated in distilled water for 0–48 h at 37°C. TRL 1215 cells were exposed to various concentrations of these preincubated DMAsIIIG for 48 h at 37°C, and cellular viability was then assessed by the AlamarBlue assay. (b) DMAsIIIG was preincubated in distilled water for 0–48 h at −85, 4, 22 or 37°C. TRL 1215 cells were exposed to 500 nM of these preincubated DMAsIIIG for 48 h at 37°C, and cellular viability was then assessed by the AlamarBlue assay. All results are expressed as the arithmetic mean±s.e.m. of three separate experiments each performed in triplicate (n=9). *P<0.001, in comparison to the cells exposed to the same concentrations of DMAsIIIG without preincubation, **P<0.01, ***P<0.05.