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. 2006 Oct 16;149(7):888–897. doi: 10.1038/sj.bjp.0706899

Figure 8.

Figure 8

Effects of GSH, HS or HSA on the chemical form of DMAsIIIG. DMAsIIIG (10 mM) was incubated with or without 10 mM GSH, 50% HS, or 50 g l−1 HSA for 6 or 48 h at 37°C. After incubation, aliquots (5 μl) of these mixtures were spotted onto an HPTLC plate that was developed using ethyl acetate:acetic acid:water (3:2:1), and the separated spots were detected with iodine vapor. Lane 1, 10 mM GSH; lane 2, 10 mM GSSG; lane 3, 10 mM DMAsV; lane 4, 10 mM DMAsIIIG; lanes 5–6, 10 mM DMAsIIIG preincubated in distilled water for 6 (lane 5) or 48 h (lane 6) at 37°C; lanes 7–8, 10 mM DMAsIIIG incubated with 10 mM GSH for 6 h (lane 7) or 48 h (lane 8) at 37°C; lane 9, 50% HS; lanes 10–11, 10 mM DMAsIIIG incubated with 50% HS for 6 h (lane 10) or 48 h (lane 11) at 37°C; lane 12, 50 g l−1 HSA; lanes 13–14, 10 mM DMAsIIIG incubated with 50 g l−1 HSA for 6 h (lane 13) or 48 h (lane 14) at 37°C.