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. Author manuscript; available in PMC: 2007 Oct 10.
Published in final edited form as: J Mol Biol. 2005 Sep 23;352(3):621–628. doi: 10.1016/j.jmb.2005.07.020

Figure 4.

Figure 4

The β-galactosidase and β-fucosidase activities of selected lacZ variants. E. coli cells were transformed with mutated 6his-lacZ-pET28a+ plasmids (or with the ancestral or no insert control plasmids), grown to mid-log stage in LB-kanamycin liquid cultures, and induced for 3 hours with 0.5 mM IPTG. Each culture was split and reacted with either 0.5 mM para-nitrophenyl-β-d-galactopyranoside (native substrate, red) or para-nitrophenyl-β-d-fucopyranoside (novel substrate, blue). The formation of the para-nitrophenol product was monitored continously in a microplate spectrophotometer for 16 h.