Table 1.

Primers used in this study

Primer name	Sequencea	Use
lacZ D201X	ATCTGGAAGATCAGNNKATGTGGCGGATGA	PCR 1: randomize D201
lacZ D201X rev	TCATCCGCCACATMNNCTGATCTTCCAGATA	PCR 4: randomize D201
lacZ H540X	TTGCGAATACGCCNNKGCGATGGGTAACA	PCR 2: randomize H540
lacZ H540X rev	TGTTACCCATCGCMNNGGCGTATTCGCAAAG	PCR 1: randomize H540
lacZ N604X	CCAGTTCTGTATGNNKGGTCTGGTCTTTG	PCR 3: randomize N604
lacZ N604X rev	CAAAGACCAGACCMNNCATACAGAACAGGC	PCR 2: randomize N604, adds Q600L mutation
3′ pET3	Phosphate-GTCGACGTTGGAGTCCACGTTCTTTAATA	PCR 3 and 5: amplify whole plasmid
3′ pETout2	Phosphate-GCATGCCGTAAAGCACTAAATCGGAACC	PCR 4 and 5: amplify whole plasmid
lacZ 454 rev	CGACCCAGCGCCCGTTGCACCACAG	lacZ sequencing
lacZ 377	AATCCGACGGGTTGTTACTCGCTCAC	lacZ sequencing
lacZ 770	GAGTTGCGTGACTACCTACGGGTAAC	lacZ sequencing
lacZ 1154	CTGAACGGCAAGCCGTTGCTGATTC	lacZ sequencing
lacZ 1536	CGCGTGGATGAAGACCAGCCCTTC	lacZ sequencing
lacZ 1920	CGGGCAAACCATCGAAGTGACCAGC	lacZ sequencing
lacZ 2269	CATCGAGCTGGGTAATAAGCGTTGGC	lacZ sequencing
lacZ 2679	TGCCAGCTGGCGCAGGTAGCAGAG	lacZ sequencing

^aK = G or T; M = C or A.