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Sequences of most active β-fucosidases
| N147G aac → ggc | D164Y g490t | H540V cac → gtt | N600V a1799t | N604T aac → act | |
|---|---|---|---|---|---|
| 1 | X | X | X | ||
| 5 | X | X | X | ||
| 10 | X | X | X | ||
| 13 | X | X | X | ||
| 18 | X | X | X | ||
| 22 | X | X | X | X | |
| 26 | X | X | X | ||
| 29 | X | X | X | X |
Clone 26 was sequenced completely, the others were sequenced for nucleotides 400–1000 and 1600–2100 (wild-type lacZ numbering). The ancestral and selected lacZ variants all contain the silent t570c mutation. The Q600V mutation was accidentally encoded in the lacZ N604T rev primer.
