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. Author manuscript; available in PMC: 2007 Oct 10.

Published in final edited form as: J Mol Biol. 2005 Sep 23;352(3):621–628. doi: 10.1016/j.jmb.2005.07.020

Table 3.

Steady-state kinetic parameters of BGAL variants

	pNP-gal			pNP-fuc
	k_cat (s⁻¹)	K_M (μM)	k_cat/K_M (s⁻¹ M⁻¹)	k_cat (s⁻¹)^a	K_M (μM)	k_cat/K_M
Wild-type	33.4 ± 3.7	48.0 ± 5.4	723,000 ± 143,000		> 3000	47 ± 3
H540V	1.0 ± 0.0	916.9 ± 75.3	1114 ± 111		> 3000	10,610 ± 4516
N604T	4.1 ± 0.1	64.5 ± 3.6	64,928 ± 6,084		> 3000	110 ± 4
H540V/N604T	0.1 ± 0.0	702.0 ± 22.9	179 ± 2	21.5 ± 2.4	2563.1 ± 78.6	8365 ± 735

^a

The wild-type, H54-V and N604T BGAL variants could not be saturated with pNP-fuc, so neither the k_cat nor K_M value could be determined accurately.