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. Author manuscript; available in PMC: 2007 Oct 10.
Published in final edited form as: J Biol Chem. 2006 Dec 22;282(8):5367–5377. doi: 10.1074/jbc.M610993200

FIGURE 7. Effect of the T455A mutation on human CTP synthetase activity, and on the cellular concentration of CTP in S. cerevisiae.

FIGURE 7

A, the wild type (WT) and T455A mutant human CTP synthetase 1 enzymes were expressed and purified from S. cerevisiae ura7Δ ura8Δ cells. CTP synthetase activity was determined with the spectrophotometric assay, and the activity of the wild type enzyme was set at 100%. B, S. cerevisiae ura7Δ ura8Δ cells expressing the wild type and T455A mutant human CTP synthetase 1 enzymes were grown to the exponential phase of growth. Nucleotides were extracted and the concentration of CTP was analyzed by high performance liquid chromatography. The values reported were average of three separate experiments ± S.D.