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. Author manuscript; available in PMC: 2007 Oct 10.
Published in final edited form as: J Biol Chem. 2006 Dec 22;282(8):5367–5377. doi: 10.1074/jbc.M610993200

TABLE 1.

Strains used in this work

Strain Relevant characteristics Source or Ref.
E. coli
 DH5α F−, φ80dlacZΔM15, Δ(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(rk− mk+), phoA, supE44, λ−thi-1, gyrA96, relA1 (53)
S. cerevisiae
 SDO195 MATa leu2-3,112 trp1-289 ura3-52 ura7Δ::TRP1 ura8Δ::hisG [pDO134] (19)
 GHY55 MATa leu2-3,112 trp1-289 ura3-52 ura7Δ::TRP1 ura8Δ::hisG [pDO105-hCTPS1] (40)
 MCY24 MATa leu2-3,112 trp1-289 ura3-52 ura7Δ::TRP1 ura8Δ::hisG [pDO105-hCTPS1(T83A)] This study
 MCY20 MATa leu2-3,112 trp1-289 ura3-52 ura7Δ::TRP1 ura8Δ::hisG [pDO105-hCTPS1(S219A)] This study
 MCY25 MATa leu2-3,112 trp1-289 ura3-52 ura7Δ::TRP1 ura8Δ::hisG [pDO105-hCTPS1(T378A)] This study
 MCY26 MATa leu2-3,112 trp1-289 ura3-52 ura7Δ::TRP1 ura8Δ::hisG [pDO105-hCTPS1(T455A)] This study
 MCY21 MATa leu2-3,112 trp1-289 ura3-52 ura7Δ::TRP1 ura8Δ::hisG [pDO105-hCTPS1(S552A)] This study
 MCY22 MATa leu2-3,112 trp1-289 ura3-52 ura7Δ::TRP1 ura8Δ::hisG [pDO105-hCTPS1(S562A)] This study
 MCY27 MATa leu2-3,112 trp1-289 ura3-52 ura7Δ::TRP1 ura8Δ::hisG [pDO105-hCTPS1(T566A)] This study
 MCY23 MATa leu2-3,112 trp1-289 ura3-52 ura7Δ::TRP1 ura8Δ::hisG [pDO105-hCTPS1(S573A)] This study
 MCY28 MATa leu2-3,112 trp1-289 ura3-52 ura7Δ::TRP1 ura8Δ::hisG [pDO105-hCTPS1(T455A,S552A)] This study