TABLE 2.
Plasmids used in this work
| Plasmid | Relevant characteristics | Source or Ref |
|---|---|---|
| YEpLac181 | Multicopy E. coli/yeast shuttle vector with LEU2 | (83) |
| YEpLac195 | Multicopy E. coli/yeast shuttle vector with URA3 | (83) |
| pDO105 | Derivative of YEpLac181 with the ADH1 promoter and multiple cloning sites | (19) |
| pDO134 | URA7 derivative of YEpLac195 | (19) |
| pDO105-hCTPS1 | CTPS1 derivative of pDO105 | (40) |
| pDO105-hCTPS1(T83A) | CTPS1T83A derivative of pDO105-hCTPS1 | This study |
| pDO105-hCTPS1(S219A) | CTPS1S219A derivative of pDO105-hCTPS1 | This study |
| pDO105-hCTPS1(T378A) | CTPS1T378A derivative of pDO105-hCTPS1 | This study |
| pDO105-hCTPS1(T455A) | CTPS1T455A derivative of pDO105-hCTPS1 | This study |
| pDO105-hCTPS1(S552A) | CTPS1S552A derivative of pDO105-hCTPS1 | This study |
| pDO105-hCTPS1(S562A) | CTPS1S562A derivative of pDO105-hCTPS1 | This study |
| pDO105-hCTPS1(T566A) | CTPS1T566A derivative of pDO105-hCTPS1 | This study |
| pDO105-hCTPS1(S573A) | CTPS1S573A derivative of pDO105-hCTPS1 | This study |
| pDO105-hCTPS1(T455A,S552A) | CTPS1T455A,S552A derivative of pCTPS T455A | This study |