TABLE 3.

Oligonucleotides used in this work

Oligo	Sequence
T83A Fa	5′-CTTGACATCCGCCTCGCCAAGGACAATAATCTG-3′
T83A Rb	5′-CAGATTATTGTCCTTGGCGAGGCGGATGTCAAG-3′
S219A F	5′-GTTGTATGCAGGTGCGCAAATCCACTTGACAC-3′
S219A R	5′-GTGTCAAGTGGATTTGCGCACCTGCATACAAC-3′
T378A F	5′-GATTTGGTGTTCGAGGAGCAGAAGGAAAAATCC-3′
T378A R	5′-GGATTTTTCCTTCTGCTCCTCGAACACCAAATC-3′
T455A F	5′-CTGGGCAAGAGGAGAGCCCTGTTCCAGACCAAG-3′
T455A R	5′-CTTGGTCTGGAACAGGGCTCTCCTCTTGCCCAG-3′
S552A F	5′-CTGTGGGGCGGCTCGCACATTACCTCCAG-3′
S552A R	5′-CTGGAGGTAATGTGCGAGCCGCCCCACAG-3′
S562A F	5′-GAAAGGCTGCAGGCTCGCACCCAGGGACACCTATAG-3′
S562A R	5′-CTATAGGTGTCCCTGGGTGCGAGCCTGCAGCCTTTC-3′
T566A F	5′-CTCTCACCCAGGGACGCCTATAGTGACAGG-3′
T566A R	5′-CCTGTCACTATAGGCGTCCCTGGGTGAGAG-3′
S573A F	5′-GTGACAGGAGTGGAGCCAGCTCCCCTGACTC-3′
S573A R	5′-GAGTCAGGGGAGCTGGCTCCACTCCTGTCAC-3′

^aForward orientation relative to sense strand

^bReverse orientation relative to sense strand