Table 1.

Cloning efficiency when using Sticky RICE method

Insert size (bp)	5'PO4 on insert	Enzyme mixa	Reaction time	# of colonies	% with insertb
770	No	Pol/Kin/Lig	30 min	77	~90%
770	Yes	Pol/Lig	30 min	365	~90%

^aPol, 0.25 Units (U) T4 DNA polymerase; Kin, 0.5 U T4 polynucleotide kinase; Lig, 400 U T4 DNA ligase.

^bdetermined by restriction endonuclease screening of plasmids (n = 8)