Table 3.
Primer set | Name of primer | PCR round | Sequence 5'- 3' | Product length $ (bp) |
RH-gB | 2759s | 1 | CCTCCCAGGTTCARTWYGCMTAYGA | 700 |
2762as | CCGTTGAGGTTCTGAGTGTARTARTTRTAYTC | |||
2760s | 2 | AAGATCAACCCCAC(N/I#)AG(N/I)GT(N/I)ATG | 500 | |
2761as | GTGTAGTAGTTGTACTCCCTRAACAT(N/I)GTYTC | |||
LC-gB | 2753s | 1 | CCATCCAGATCCARTWYGC(N/I)TAYGA | 650 |
2756as | GATGTTCTGCGCCTRRWARTTRTA | |||
2754s | 2 | TGGCTGCCAAGCG(N/I)(N/I)T(N/I)GG(N/I)GA | 460 | |
2755as | GATGTTCTGCGCCTGRWARTTRTAYTC | |||
CM-gB | 2743s | 1 | CGCAAATCGCAGA(N/I)KC(N/I)TGGTG | 330 |
2746as | TGGTTGCCCAACAG(N/I)ATYTCRTT | |||
2744s | 2 | TTCAAGGAACTCAGYAARAT(N/I)AAYCC | 250 | |
2745as | CGTTGTCCTC(N/I)CC(N/I)ARYTG(N/I)CC | |||
CM-MDBP | 3730s | 1 | TGTGGCTTCTCATGCTTvCA[n/i]TT[n/i]TG | 560 |
3730as | GTTGAGGCTCCG[n/i]TCsAC[n/i]CC | |||
3731s | 2 | CTATCTCGAGCATCG[n/i]TTyCAyAAC | 350 | |
3731as | AAAAGTACCCAATCTG[n/i]CCrAAsTG |
# I = Inosine
$ approximate values