Table 4.
Amplification of an under-represented LCV species in the presence of two over-represented, closely related LCV species
| Viral templates added to the PCR reaction | Copy number of added template | Pan-herpes PCR detection limit (template copy number) | Ratio between added template concentration and the detection limit | LNA added | Expected virus detection | Detected virus | ||
| A | 1 | EBV | 10.000 | 10.000 | 1 | - | ||
| 2 | GgorLCV1 | 100 | 10 | 10 | GgorLCV1 | EBV | EBV | |
| 3 | CalHV3 | 10 | 1 | 10 | CalHV3 | |||
| B | 1 | EBV | 100.000 | 10.000 | 10 | EBV | ||
| 2 | GgorLCV1 | 10 | 10 | 1 | - | GgorLCV1 | GgorLCV1 | |
| 3 | CalHV3 | 10 | 1 | 10 | CalHV3 | |||
| C | 1 | EBV | 100.000 | 10.000 | 10 | EBV | ||
| 2 | GgorLCV1 | 100 | 10 | 10 | GgorLCV1 | CalHV3 | CalHV3 | |
| 3 | CalHV3 | 1 | 1 | 1 | - | |||
| D | 1 | EBV | 10.000 | 10.000 | 1 | - | ||
| 2 | GgorLCV1 | 1000 | 10 | 100 | GgorLCV1 | EBV | EBV | |
| 3 | CalHV3 | 100 | 1 | 100 | CalHV3 | |||
| E | 1 | EBV | 1000.000 | 10.000 | 100 | EBV | ||
| 2 | GgorLCV1 | 10 | 10 | 1 | - | GgorLCV1 | GgorLCV1 | |
| 3 | CalHV3 | 100 | 1 | 100 | CalHV3 | |||
| F | 1 | EBV | 1000.000 | 1.000 | 100 | EBV | ||
| 2 | GgorLCV1 | 1000 | 10 | 100 | GgorLCV1 | CalHV3 | GgorLCV1 | |
| 3 | CalHV3 | 1 | 1 | 1 | - |