Table 1.

Summary of two bacterial artificial chromosome (BAC) libraries

	EcoRI-digested library	BamHI-digested library
Vector	pBACe3.6 [52]	pBeloBAC11 [53]
Cloning site	EcoRI	BamHI
Number of clones	36000 (96 × 384 wells)	21120 (55 × 384 wells)
Mean insert size (kbp)	168	165
Clone coverage	× 11.4	× 6.6
Strain	p50T (mixed insects)	p50T (mixed insects)

To calculate the percentage of the silkworm genome covered by the clones (clone coverage) in the EcoRI- and BamHI-digested libraries, we assumed that the silkworm genome size was 530 Mb [34]. Detailed information on the EcoRI-digested library, such as the size distribution of BAC inserts, is available in the paper cited [49] and at the RPCI-96 BAC Library website [32]. Detailed information on the BamHI-digested library can be obtained from the website of Texas A&M BAC Libraries [33].