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. 2007 Oct 12;3(10):e146. doi: 10.1371/journal.ppat.0030146

Figure 1. HMBA Transiently Disrupts the LC.

Figure 1

(A) U1, Ach-2, and JΔK cells were stimulated with increasing concentrations of HMBA (1, 5, 10, and 20 mM) and viral production was assessed at 48 h in the supernatant using p24 ELISA.

(B) Resting CD4+ T cells, which were isolated from PBMCs that were infected with HIV-1LAI and rested for 11 d were treated or not with HMBA (1 mM) for 3 d. Viral production was assessed using p24 ELISA.

(C) Total cell lysates of Jurkat cells stimulated or not with HMBA (5 mM) for 30 min, 1 h, 2 h, 6 h, and 24 h, were subjected to glycerol gradient sedimentation analyses (10%–30%), and the fractions were analyzed by western blotting using HEXIM1, HEXIM2, and Cdk9 antibodies. Numbers below the western blots correspond to fractions from glycerol gradient analyses.

(D) LC/SC represents the ratio of cumulated intensities of fractions 5, 6, and 7 (LC) over intensities of fractions 1, 2, and 3 (SC). Values were normalized to ratios obtained in unstimulated cells.