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. 2007 Oct 12;3(10):e146. doi: 10.1371/journal.ppat.0030146

Figure 6. T270 and S278 in the TBD of HEXIM1 Are Phosphorylated following the Activation of Akt In Vivo.

Figure 6

f:Hex1 protein and mutant f:Hex1(4A) protein in (A), and mutant f:Hex1(268A), f:Hex1(270A), f:Hex1(276A), or f:Hex1(278A) proteins in (B) were expressed in Jurkat cells transfected with an empty plasmid vector or a vector expressing M-Akt. After 24 h, protein extracts were subjected to immunoprecipitation using anti-Flag antibodies, and levels of phospho-Akt substrates (pAktSub) and Flag-tagged expressed proteins were measured by western blotting.