Skip to main content
. 1997 Mar 18;94(6):2676–2680. doi: 10.1073/pnas.94.6.2676

Figure 4.

Figure 4

Regulation of iNOS expression by constitutive NOS. (A) Western blot analysis indicates that PDTC (100 μM) dramatically induces iNOS expression in glial cultures with iNOS protein being detectable at 4 h and peaking at 8 h after PDTC treatment. PC, positive control. (B) PDTC (100 μm) alone significantly suppresses basal nitrite formation compared with control (∗, P < 0.05). PDTC also significantly inhibits LPS (24 h)-stimulated nitrite formation compared with LPS alone (∗∗, P < 0.01). Results represent the mean of five independent determination ± SD. (C) Northern blot analysis shows that PDTC did not affect the level of iNOS mRNA induced by LPS (24 h). (D) Western blot analysis shows that PDTC did not affect the level of iNOS protein induced by LPS (24 h). A, C, and D are representative of at least three independent experiments.