Table 1 Antibodies used in study with sources, dilutions and methods of pretreatment.

Antibody	Source	Dilution	Pretreatment
AE1/3	Dako (Ely, UK)	1:100	Protease 1 (4 min)
Epithelial membrane antigen	Dako	1:10	See materials and methods
Desmin	Dako	1:100	See materials and methods
α smooth muscle actin	Dako	1:500	See materials and methods
h‐caldesmon	Dako	1:50	Pressure cooker 2 min, pH 7.8 buffer; protease 3 (2 min)
α inhibin	Dako	1:200	See materials and methods
Calretinin	Zymed (San Francisco, CA, USA)	1:50	See materials and methods
Melan A	Dako	1:10	See materials and methods
CD99	Dako	1:10	None
Oestrogen receptor (clone sp1)	Labvision (Fremont, CA, USA)	1:200	See materials and methods
Progesterone receptor	Dako	1:50	See materials and methods
Androgen receptor	Novocastra (Newcastle, UK)	1:100	See materials and methods
Vimentin	Dako	1:400	See materials and methods
WT1	Dako	1:50	See materials and methods
CD10	Novocastra	1:5	See materials and methods
CD56	Novocastra	1:50	See materials and methods
Chromogranin	Dako	1:600	See materials and methods
HMB45	Dako	1:100	See materials and methods