Table 1 Primers for RT‐PCR.
Gene | Genbank no | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) | Product size |
---|---|---|---|---|
Foxp3 | NM014009 | TCATCCGCTGGGCCATCCTG | GTGGAAACCTCACTTCTTGGTC | 385bp |
IL‐10 | NM000572.2 | ATGCCCCAAGCTGAGAACCAAGACCCA | TCTCAAGGGGCTGGGTCAGCTATCCCA | 352bp |
TGFβ | NM000660.3 | CAGAAATACAGCAACAATTCCTGG | TTGCAGTGTGTTATCCCTGCTGTC | 190bp |
TNFα | NM000594.2 | GAGTGACAAGCCTGTAGCCCATGTTGTAGCAT | GCAATGATCCCAAAGTAGACCTGCCCAGACT | 444bp |
IFNγ | NM000619.2 | GCATCGTTTTGGGTTCTCTTGGCTCTTACTGC | CTCCTTTTTCGCTTCCCCTGTTTTAGCTGCTGG | 427bp |
G3PDH | BC023632 | GAACGGGAAGCTCACTGGCATGGC | TGAGGTCCACCACCCTGTTGCTG | 311bp |