. Author manuscript; available in PMC: 2008 Jun 15.

Published in final edited form as: Toxicol Appl Pharmacol. 2007 Mar 30;221(3):349–362. doi: 10.1016/j.taap.2007.03.023

Table 2. Kinetics of GSH conjugation of TRI in rat kidney cytoplasm from control and Hg²⁺-pretreated rats.

Male Fischer 344 rats were given an ip injection of either saline (Con, control) or 0.5 μmol HgCl₂/kg (Hg²⁺). After 24 or 48 h, cytoplasm was isolated from kidney cortex of each pretreatment group and were incubated with 5 mM GSH and 0.2 to 10 mM TRI for up to 60 min. Kinetic parameters are derived from Eadie-Hofstee (E-H) plots of the V vs. S data shown in Fig. 2.

	E-H regression equation; R²	V_max (nmol DCVG/60 min per mg)	K_m (mM TRI)
Control-24 h	Y=3.490-0.762X; R²=0.831	4.58	1.31
Hg²⁺-24 h	Y=6.045-0.852X; R²=0.936	7.10	1.17
Control-48 h	Y=3.086-0.616X; R²=0.799	5.01	1.62
Hg²⁺-48 h	Y=5.744-0.857X; R²=0.946	6.70	1.17

Table 2. Kinetics of GSH conjugation of TRI in rat kidney cytoplasm from control and Hg2+-pretreated rats.

Table 2. Kinetics of GSH conjugation of TRI in rat kidney cytoplasm from control and Hg²⁺-pretreated rats.